use speclib\st12nds.mat, deleting the inappropriate first.
usually delete lys, his, ??
cys if no disulfide.

n:normalized. S:shifted. D derivatives.


11060301 -running spectra on oxidase-containing fractions from biogel-A (from heather) 110526
11070101 - # running spectra on oxidase that was frozen-80
11071101- Spectra of undil lysozyme: 
11071401- scanning spectra for oxidase: 
11071501-running spectra on a- Chymotrypsinogen A: 
11071801:running spectra on N- acetyl tryptophanamide and L-cystine: 
11071901: runninh spectra on ribonuclease A: 
11072001: running spectra on poly-L-lysine:
11072501: running spectra on mitos: EXTINCTION COEFFIFICENT OF MITOS IN SDS IS 0.961 PER 1 g/l
11072701: running spectra on  a- Chemotrypsinogen A and ribonuclease A w/urea: 
11072901: continuation of 110727 spectra of chymotrypsinogen and ribonuclease w/urea:
11080301: running spectra on L-tryosine:
11080401: running spectra w/guanindine hydrochloride: (and wo): RNase, CHTsgn, LZ, BSA
11081101: running spectra on L-Histidine: 
11082301: running spectra on L-phenylalanine: 
11122101: running spectra of following proteins before and after addition of guanidine HCl:
	trypsin, glucose isomerase, "protease"
11122701: running spectra on following proteins: 
	beta-galactidose (beautiful spectrum), glutamic oxalacetic transminase (turbidity)
11122801: running spectra on following protein: 
	glutamic oxalacetic transminase* (spun down and dissolved in water?)


120709 has amino acid spectra to 200 nm, for "end absorbance"
    Only tyrosine and histidine, poylysine, polyarginine, trypsin onscale below 210.
	His offscale below 220
    Polylys 100 ul and polyasn 50 ul are almost identical down to 228 nm, good for      peptide absorbance. Polyasn 100 also good but 700 has weird stuff
polylys undil was apparently 1/.128 mM - we scaled by .128 for 1 mM
Then EmM at 230 nm
120709 polylys 20 ul/700 gave A200= 1.8. *50/.128 = 391 mM-1 ?

120724 has proteins to 200 nm, but offscale below ~220
	no this is Q extraction!
120821 - hemochrome?



*NOT glutamic oxalacetic transminase:
>gi|2392158|pdb|1AJS|A Chain A, Refinement And Comparison Of The Crystal Structures Of Pig Cytosolic Aspartate Aminotransferase And Its Complex With 2-Methylaspartate
APPSVFAEVPQAQPVLVFKLIADFREDPDPRKVNLGVGAYRTDDCQPWVLPVVRKVEQRIANNSSLNHEY
LPILGLAEFRTCASRLALGDDSPALQEKRVGGVQSLGGTGALRIGAEFLARWYNGTNNKDTPVYVSSPTW
ENHNGVFTTAGFKDIRSYRYWDTEKRGLDLQGFLSDLENAPEFSIFVLHACAHNPTGTDPTPEQWKQIAS
VMKRRFLFPFFDSAYQGFASGNLEKDAWAIRYFVSEGFELFCAQSFSKNFGLYNERVGNLTVVAKEPDSI
LRVLSQMQKIVRVTWSNPPAQGARIVARTLSDPELFHEWTGNVKTMADRILSMRSELRARLEALKTPGTW
NHITDQIGMFSFTGLNPKQVEYLINQKHIYLLPSGRINMCGLTTKNLDYVATSIHEAVTKIQ


Todo:
compare tyrosinamide with prtoeins having no tryptophan
likewise for trp

It is likely that the absorption comprises multiple electronic transitins which may not respond in the same way to medium polarity.

calculate for something like bsa using known concentration,not fitting, 
and see how they differ. May give a clue which standard is problematic.

even neglecting fine structure, tyrosine and tryptophan are very different.
Plotted at same peak height, trp is much broader.