running spectra on ubiquinone extraction(upper layers) for tubes 1,2,3 using pig heart mitochondria as sample:

12072401: water baseline
12072402: 700 ul tube 1 upper layer
12072403:r
12072404:r 2
12072405: 700 ul tube 2 upper layer
12072406:r
12072407:700 ul tube 3 upper layer
12072408:r
12072409:r
12072410: flow thru tube 2
12072411:r
12072412: tube 3 hexane wash with 1mL methanol
12072413:r
12072414: tube 4 chloroform wash with 1mL methanol 
12072415:r